Investigating Spontaneous Generation Lab Investigation (Product Code: SLE/EB/17) is a microbiology class activity used to recreate Louis Pasteur's 1864 experiment and test whether life can arise from non-life in school biology labs. Students compare organisms grown in flasks open to airborne particles with flasks that have special S-shaped necks, and the activity is designed for a class of 40. It is supplied by Jain Scientific Equipments Pvt Ltd (ScienceLab), Ambala, India, as a microbiology teaching kit that joins lab skills with the history of science.
| Parameter | Detail |
|---|---|
| Experiment | Recreation of Pasteur's classic spontaneous generation experiment |
| Question investigated | Can life arise from non-life? |
| Test flasks | Flasks exposed to particles in the atmosphere |
| Comparison flasks | Flasks with special S-shaped necks, exposed to air but not to particulate matter |
| Principle shown | Microorganisms do not develop spontaneously in a sterilised nutrient broth |
| Historical context | Louis Pasteur dispelled spontaneous generation in 1864 |
| Class size | 40 students |
| Product code | SLE/EB/17 |
| Supplied by | Jain Scientific Equipments Pvt Ltd (ScienceLab), Ambala, India |
It is used to let students test one of biology's oldest questions with their own eyes. Nutrient broth is sterilised in both kinds of flask, which are then left side by side while students check them regularly for cloudiness, the usual sign of microbial growth. The open flasks, reached by dust and airborne particles, cloud over; the S-neck flasks, where particles settle in the bend of the neck, stay clear even though air can still reach the broth. Students record their observations in a table and explain the difference.
The discussion that follows is the real payoff. Students see why Pasteur's design answered the critics who said boiled broth lacked “air”, and why the result supports the idea that microbes come only from other microbes, giving firsthand insight into ideas that underpin evolution. As a bacteria culture experiment it also introduces sterilisation, contamination and controls — ideas students will need in every later microbiology practical.
ScienceLab is how Jain Scientific Equipments Private Limited presents its range; the company is based in Ambala, Haryana, India, and supplies this investigation to schools, colleges and distributors. Buyers completing vendor forms can list the supplying company under Importer-Exporter Code AAECJ8618A (DGFT) and GSTIN 06AAECJ8618A1ZT. Visit the company profile for more.
Useful additions for preparing and running the flasks:
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What does the Investigating Spontaneous Generation Lab Investigation demonstrate?
The Investigating Spontaneous Generation Lab Investigation demonstrates that microorganisms in a sterilised nutrient broth do not appear spontaneously, recreating Pasteur's 1864 result that growth follows only when particles from the air can reach the broth.
What class size is the Investigating Spontaneous Generation Lab Investigation designed for?
The Investigating Spontaneous Generation Lab Investigation is designed for a class size of 40 students, so a single order covers a typical class working in groups around the open and S-neck flasks.
Are the S-neck flasks and the broth included?
Please ask us for the current contents list of the Investigating Spontaneous Generation Lab Investigation, including whether nutrient broth is supplied. The activity is built around open flasks and flasks with special S-shaped necks, exposed to air but not to particles.
How long does any nutrient broth supplied with the investigation keep?
No fixed period is stated for media used in the Investigating Spontaneous Generation Lab Investigation, because shelf life depends on the chemical, its grade and storage conditions. Ask us for the expiry date of the batch you are quoted.
How long before results can be seen?
In the Investigating Spontaneous Generation Lab Investigation, growth in the open flasks usually becomes visible within a few days, depending on room temperature; keeping the flasks in an incubator can speed this up.
Is the practical safe for schools?
Yes, the Investigating Spontaneous Generation Lab Investigation is suitable for schools with normal microbiology hygiene: avoid opening or tipping the flasks once growth appears, and sterilise all cultures before disposal.
Who supplies the Investigating Spontaneous Generation Lab Investigation?
Jain Scientific Equipments Pvt Ltd, trading as ScienceLab from Ambala, Haryana, India, supplies the Investigating Spontaneous Generation Lab Investigation to schools and colleges in India and international markets, and quotes directly for single classes or several schools.
To bring Pasteur's experiment into your lab, request a quotation from us or add the investigation to the enquiry cart.
Last updated: 24 September 2026